Protein Details: Acid-sensing ion channel 3

Protein ID

ICDB_Pro_0208

Protein Name

Acid-sensing ion channel 3

Gene Name

Asic3; Accn3; Drasic

Organism

Rattus norvegicus (Rat)

Length

533 amino acids

AlphaFoldDB

AF-O35240-F1-model_v4.pdb

Function

Cation channel with high affinity for sodium;which is gated by extracellular protons and inhibited by the diuretic amiloride. Generates a biphasic current with a fast inactivating and a slow sustained phase. In sensory neurons is proposed to mediate the pain induced by acidosis that occurs in ischemic;damaged or inflamed tissue. May be involved in hyperalgesia. May play a role in mechanoreception. Heteromeric channel assembly seems to modulate channel properties.

Sequence

MKPRSGLEEAQRRQASDIRVFASSCTMHGLGHIFGPGGLTLRRGLWATAVLLSLAAFLYQVAERVRYYGEFHHKTTLDERESHQLTFPAVTLCNINPLRRSRLTPNDLHWAGTALLGLDPAEHAAYLRALGQPPAPPGFMPSPTFDMAQLYARAGHSLEDMLLDCRYRGQPCGPENFTVIFTRMGQCYTFNSGAHGAELLTTPKGGAGNGLEIMLDVQQEEYLPIWKDMEETPFEVGIRVQIHSQDEPPAIDQLGFGAAPGHQTFVSCQQQQLSFLPPPWGDCNTASLDPDDFDPEPSDPLGSPRPRPSPPYSLIGCRLACESRYVARKCGCRMMHMPGNSPVCSPQQYKDCASPALDAMLRKDTCVCPNPCATTRYAKELSMVRIPSRASARYLARKYNRSESYITENVLVLDIFFEALNYEAVEQKAAYEVSELLGDIGGQMGLFIGASLLTILEILDYLCEVFQDRVLGYFWNRRSAQKRSGNTLLQEELNGHRTHVPHLSLGPRPPTTPCAVTKTLSASHRTCYLVTRL

PDB Structures

Ligand Binding

1. DICL_CP

2. DICL_Pep

Binding Site

SITE 26: "Potassium ion selectivity and permeability"

Disease

pain induced by acidosis that occurs in ischemic; damaged or inflamed tissue; hyperalgesia

Location

Expressed in sciatic nerve and dorsal root ganglion (at protein level). Expressed in sensory neurons of dorsal root ganglion. Expressed in Golgi interneurons in the granular layer. Expressed in Golgi interneurons in the granular layer. Expressed in Golgi interneurons in the granular layer. Also found in superior cervical ganglia;spinal cord and brain stem

DOI ID

10.1074/jbc.272.34.20975; 10.1074/jbc.272.47.29778; 10.1073/pnas.95.17.10240; 10.1074/jbc.274.15.10129; 10.1074/jbc.m004114200; 10.1523/jneurosci.21-20-08026.2001; 10.1038/nn0901-869; 10.1016/s0028-3908(01)00107-1; 10.1073/pnas.98.2.711; 10.1523/jneurosci.22-24-10662.2002; 10.1073/pnas.042688199; 10.1074/jbc.m309468200; 10.1016/s0896-6273(02)01130-3; 10.1016/s0028-3908(03)00047-9; 10.1038/sj.emboj.7600177; 10.1074/jbc.m313078200; 10.1074/jbc.m405874200; 10.1038/emboj.2012.203; 10.1073/pnas.1616232114

RefSeq

NP_775158.1